배아줄기세포 추출액을 이용한 신경 줄기세포 리프로그래밍 연구로서 우선, 리프로그래밍 원료 단백질 추출을 위한 기존 자료 조사를 마치고, 리프로그래밍 단백질로 사용할 배아 줄기세포 단백질 추출법 확립, 박테리아 독소 및 카이네이즈를 이용한 리프로그래밍 방법 ...

배아줄기세포 추출액을 이용한 신경 줄기세포 리프로그래밍 연구로서 우선, 리프로그래밍 원료 단백질 추출을 위한 기존 자료 조사를 마치고, 리프로그래밍 단백질로 사용할 배아 줄기세포 단백질 추출법 확립, 박테리아 독소 및 카이네이즈를 이용한 리프로그래밍 방법 수립, 5 stage 응용법 개발, 리프로그램화된 세포의 세포 생물학적 특성 분석하였다. 배아줄기세포 추출액을 이용한 신경 줄기세포 리프로그래밍로 보다 원시 상태의 세포로 그 특성을 띤다. 다음으로, 줄기세포성 유지를 위한 유전자, 과발현 바이러스를 이용한 신경 줄기세포 리프로그래밍 연구로첫째, 줄기세포성 유지를 위한 유전자의 과발현 시스템으로 레트로바이러스 제작 및 최적 발현 조건 확립, 두번 째, 리프로그래밍 세포주 확립을 위한 실제 도입 하였고, 셋째, 리프로그램 세포주의 세포 생물학적 특성 분석 연구하였다. E12 및 14 일자 신경 줄기세포 리프로그래밍로 보다 원시 상태의 세포로 그 특성을 띤다.

A critical question in stem cell science/therapy is whether it is possible to reprogram and/or dedifferentiate adult stem/precursor cells to a less specialized cell state with more potent proliferative and differentiation capacities. Toward this goal, ...

A critical question in stem cell science/therapy is whether it is possible to reprogram and/or dedifferentiate adult stem/precursor cells to a less specialized cell state with more potent proliferative and differentiation capacities. Toward this goal, different strategies have been attempted by numerous laboratories, including nuclear transfer, cellular fusion, the use of cell extracts, culture-induced and distinct factors-induced reprogramming. Based on recent studies demonstrating that most, if not all, of reprogramming activities reside in embryonic stem cells (ESC), we speculated that cell-free systems (e.g., cell extracts) of ESC have great potential to study reprogramming. To address this possibility, we tested cell extracts from J1 mouse ESC and those from 293 cells as the control for reprogramming activities. We optimized the conditions of permeabilization of E14 rat cortical neural precursors with streptolysin-O (SLO). Permeabilized neural precursors were exposed to J1 ESC extracts and the defined clones were selected and analyzed. We found that, compared to 293 cell extracts, J1 ESC extracts were indeed able to induce certain biochemical/molecular aspects of reprogramming. For instance, these reprogrammed cortical neural precursors maintained their ability to differentiate into neurons even after long-term passages and maintenance (>one month) while the control cells totally lost this ability. Our results indicate that reprogramming of adult cells/procursors by ESC extracts may represent a promising approach to prepare useful cell resourse for therapeutic purposes.

A critical question in stem cell science/therapy is whether it is possible to reprogram and/or dedifferentiate adult stem/precursor cells to a less specialized cell state with more potent proliferative and differentiation capacities. Toward this goal, ...

A critical question in stem cell science/therapy is whether it is possible to reprogram and/or dedifferentiate adult stem/precursor cells to a less specialized cell state with more potent proliferative and differentiation capacities. Toward this goal, different strategies have been attempted by numerous laboratories, including nuclear transfer, cellular fusion, the use of cell extracts, culture-induced and distinct factors-induced reprogramming. Based on recent studies demonstrating that most, if not all, of reprogramming activities reside in embryonic stem cells (ESC), we speculated that cell-free systems (e.g., cell extracts) of ESC have great potential to study reprogramming. To address this possibility, we tested cell extracts from J1 mouse ESC and those from 293 cells as the control for reprogramming activities. We optimized the conditions of permeabilization of E14 rat cortical neural precursors with streptolysin-O (SLO). Permeabilized neural precursors were exposed to J1 ESC extracts and the defined clones were selected and analyzed. We found that, compared to 293 cell extracts, J1 ESC extracts were indeed able to induce certain biochemical/molecular aspects of reprogramming. For instance, these reprogrammed cortical neural precursors maintained their ability to differentiate into neurons even after long-term passages and maintenance (>one month) while the control cells totally lost this ability. Our results indicate that reprogramming of adult cells/procursors by ESC extracts may represent a promising approach to prepare useful cell resourse for therapeutic purposes.

배아 줄기세포의 세포질 내용물을 이용하여, 신경 줄기세포를 리프로그래밍된 배아 줄기세포성 세포주로 확립, 그 특성 분석 중에 있음. 향후 세포주가 확립된다면, 이를 이용하여 기본적인 발생 연구뿐만 아니라, 새로운 세포 치료제로서의 활용도 기대해 볼 수 있음.
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배아 줄기세포의 세포질 내용물을 이용하여, 신경 줄기세포를 리프로그래밍된 배아 줄기세포성 세포주로 확립, 그 특성 분석 중에 있음. 향후 세포주가 확립된다면, 이를 이용하여 기본적인 발생 연구뿐만 아니라, 새로운 세포 치료제로서의 활용도 기대해 볼 수 있음.