1. The disintegrin-metalloproteinases ADAM 10, ADAM 12 and ADAM 17 are upregulated in invading peripheral tumor cells of basal cell carcinomas.
Background: Members of the ADAM family are expressed in malignant tumours and participate in the pathoge ...
1. The disintegrin-metalloproteinases ADAM 10, ADAM 12 and ADAM 17 are upregulated in invading peripheral tumor cells of basal cell carcinomas.
Background: Members of the ADAM family are expressed in malignant tumours and participate in the pathogenesis of cancer. However, the presence of ADAM 10, 12, 17 and their role in basal cell carcinoma (BCC) have not been described. The purpose of this study was to investigate ADAM 10, 12 and 17 expression in BCC.
Methods: ADAM 10, 12, 17 expression was analyzed by immunohistochemistry in skin tissues obtained from 25 patients with different types of BCC.
Results: ADAM 10, 12, 17 immunoreactivity was increased at the peripheral tumor margin as compared to central areas of BCC tumor cell nests. Immunoreactivity of ADAM 10, 12 was increased in the deep margin of invading tumor cell nests in mixed BCC. Exceptionally immunoreactivity of ADAM 17 was increased in superficial BCC. In addition, focally increased expression of ADAM 12 was detected in squamoid differentiated tumor cells of nodular BCC.
Conclusions: ADAM 10, 12, 17 showed different expression pattern in BCC histologic subtypes, indicating their different role in the BCC pathogenesis. Overexpression of ADAM 10, 12, 17 immunoreactivity in deep invasion area of BCC, indicates that these three proteases may play an important role in the locally invasive and highly destructive growth behaviour of BCC. Additionally, we suggest that ADAM 17 may play an important role in early development of BCC. These results imply that inhibitors of ADAM 10, 12, 17 may be useful as a novel therapeutic modality.
2. Overexpression of CXCL16 in lesional psoriatic skin
Background: Psoriasis is characterized as an autoimmune disease resulting in an exaggerated innate immune response. The CXC-chemokine ligand 16 (CXCL16) is described to function as an adhesion molecule, a scavenger receptor or as a soluble molecule it acts as a chemoattractant. CXCL16 has been reported to be expressed in a variety of inflammatory diseases. However, no information has been reported in the literature about the expression of CXCL16 in psoriatic skin.
Purpose: The present study was designed to analyze the expression and localization of CXCL16 in human psoriatic skin tissues.
Materials and methods: 10 paraffinized specimens of human lesional psoriatic skin and 5 paraffinized specimens of normal skin were studied using an immunohistochemical streptavidin-peroxidase technique.
Results: In normal skin, cytpoplasmic expression of CXCL16 was increased in keratinocytes of upper epidermal cell layers as compared to the lower epidermal cell layers. In lesional psoriatic skin, CXCL16 immunoreactivity was increased in the cytoplasm of keratinocytes of lower epidermal layer kerartinocytes as compared to the normal epidermis. Cytoplasmic CXCL16 expression was increased in the capillary endothelial cells of psoriatic dermis as compared to capillary endothelial cells of the normal dermis. Notably, almost all inflammatory cells in the dermis were negative for CXCL16.
Conclusion: We here report for the first time alterations in the immunohistochemical staining pattern of CXCL16 in lesional psoriatic skin compared to the normal skin. These results suggest that CXCL16 may play a role in the pathogenesis of psoriasis.